Journal: Immunity

Article Title: Caspase-8 Collaborates with Caspase-11 to Drive Tissue Damage and Execution of Endotoxic Shock

doi: 10.1016/j.immuni.2018.06.011

Figure Lengend Snippet: (A–B) Kaplan-Meier survival plot (left) and body temperature plot (right) over time (hpc) in LPS-challenged WT (n=19), Ripk3−/− (n=15), Casp8+/−Ripk3−/− (n=8), Casp8−/−Ripk3−/− (n=26) and Casp11−/− (n=11) mice (A) or WT (n=6), Ifnar1−/− (n=8) and Irf3−/− (n=5) mice (B). (C) Kaplan-Meier survival plot for WT (n=6), littermate Casp8+/−Ripk3−/− (n=6) and Casp8−/−Ripk3−/− (n=7) as well as Casp11−/− mice (n=6) given a poly(I:C) prime (4 mg/kg) followed 6 h later with low dose LPS challenge (5 mg/kg). (D) Kaplan-Meier survival plot for WT (n=19), littermate Casp8+/−Ripk3−/− (n=20) and Casp8−/−Ripk3−/− (n=15) mice over time, h post infection (hpi) with E. coli (ATCC 25922). Groups here included balanced numbers of male and female animals. Statistical comparisons (to WT) was by Log-Rank (Mantel-Cox) test (* p<0.05; ** p<0.01). Please also see Figure S1.

Article Snippet: These data reveal a crucial requirement for Casp8 in TLR4-dependent endotoxemia beyond the recognized role of the type I IFN-Casp11-Gsdmd axis ( Kayagaki et al., 2015 ; Kayagaki et al., 2011 ). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 1 caption a7 caption a8 Casp8 drives endotoxic shock (A–B) Kaplan-Meier survival plot (left) and body temperature plot (right) over time (hpc) in LPS-challenged WT (n=19), Ripk3 −/− (n=15), Casp8 +/− Ripk3 −/− (n=8), Casp8 −/− Ripk3 −/− (n=26) and Casp11 −/− (n=11) mice (A) or WT (n=6), Ifnar1 −/− (n=8) and Irf3 −/− (n=5) mice (B). (C) Kaplan-Meier survival plot for WT (n=6), littermate Casp8 +/− Ripk3 −/− (n=6) and Casp8 −/− Ripk3 −/− (n=7) as well as Casp11 −/− mice (n=6) given a poly(I:C) prime (4 mg/kg) followed 6 h later with low dose LPS challenge (5 mg/kg). (D) Kaplan-Meier survival plot for WT (n=19), littermate Casp8 +/− Ripk3 −/− (n=20) and Casp8 −/− Ripk3 −/− (n=15) mice over time, h post infection (hpi) with E. coli (ATCC 25922).

Techniques: Infection

Journal: ACS infectious diseases

Article Title: Detection of "Hidden" Antimicrobial Drug Resistance.

doi: 10.1021/acsinfecdis.9b00132

Figure Lengend Snippet: Figure 3 Growth of E coli strains and other clinically isolated infectious bacteria: (Upper- panel A-L): growth curve tracked by EZMTT method; (Lower-panel a-l): growth curve tracked by turbidity method; doubling times at the log phase measured by the EZMTT and by the turbidity methods were A) 0.80±0.11h and 0.79±0.10 h for DH5α, respectively; B) 1.4±0.4 h and 1.6±0.3 h for MG1655 (wild type E. coli), respectively; C) 0.42±0.06 h and 0.42±0.08 h for EAEC ( adhesive E. coli), respectively; D) 0.40±0.11 h and 0.40±0.07 h for EIEC (invasive E. coli); E) 0.43±0.09 h and 0.42±0.09 h for EPEC (pathogenic E. coli), F) 0.41±0.05 h and 0.40±0.07 h for ETEC (toxigenic E. coli), respectively; G) 0.72±0.23 h and 0.74±0.06 h for Salmonella, respectively; H) 0.80±0.10 h and 0.71±0.08 h for Salmonella paratyphi, respectively; I) 1.33±0.14 h and 0.82±0.20 h for Shigella flexneri, respectively; J) 1.17±0.02 h and 1.30±0.27 h for Shigella sonnei, respectively; K) 0.50±0.07 h and 0.49±0.06 h for Staphylococcus aureus, respectively; L) 0.42±0.08 h and 0.47±0.18 h for Bacillus cereus, respectively.

Article Snippet: Mueller-Hinton Broth was purchased from OXOID(Hampshire,U.K;Chemicals from Sigma (USA), EZMTT purchased from JNF Bioscience (China; USA), CCK-8 or WST-8 from Beyotime Biotechnology (China), Enterococcus casseliflavus Collins et al. ATCC700327, Escherichia coli (Migula) Castellani and Chalmers ATCC 25922,Pseudomonas aeruginosa (Schroeter) Migula ATCC 27853,Staphylococcus aureus subsp. aureus Rosenbach ATCC 25923 were purchased from ATCC (USA).

Techniques: Isolation, Bacteria, Adhesive

Journal: ACS infectious diseases

Article Title: Detection of "Hidden" Antimicrobial Drug Resistance.

doi: 10.1021/acsinfecdis.9b00132

Figure Lengend Snippet: Figure 5. AST of antibiotics in clinically isolated A) E. coli (B) Staphylococcus aureus and (C) Bacillus cereus strains (2 10 CFU/ml) using the turbidity and the EZMTT methods; (AMP, KAN, GEN) DIP curves collected by the turbidity and the EZMTT methods; (5 hrs, 16 hrs) z-factors measured using the absorbance of OD750 nm in the turbidity assay and the absorbance of OD450 nm in the EZMTT assay at both the 5 hrs and the 16 hrs time points, respectively (z factor > 0.5 indicates excellent reproducibility; calculation z =1-[(3SD of high control)+(3SD of low control)]/[(mean of high control)-(mean of low control)]); (IC50) IC50 curves collected at the 24 hrs time point by the turbidity (black curve), the EZMTT (red curve) and the MTT methods (blue curve), showing essentially the same AST results, but the EZMTT assay showed much better assay precision.

Article Snippet: Mueller-Hinton Broth was purchased from OXOID(Hampshire,U.K;Chemicals from Sigma (USA), EZMTT purchased from JNF Bioscience (China; USA), CCK-8 or WST-8 from Beyotime Biotechnology (China), Enterococcus casseliflavus Collins et al. ATCC700327, Escherichia coli (Migula) Castellani and Chalmers ATCC 25922,Pseudomonas aeruginosa (Schroeter) Migula ATCC 27853,Staphylococcus aureus subsp. aureus Rosenbach ATCC 25923 were purchased from ATCC (USA).

Techniques: Isolation, Control

Journal: ACS infectious diseases

Article Title: Detection of "Hidden" Antimicrobial Drug Resistance.

doi: 10.1021/acsinfecdis.9b00132

Figure Lengend Snippet: Figure 6. DIP curves collected by the turbidity (OD600nm) and the EZMTT (OD450nm) methods, showing the growth inhibitions of the wild type E. coli (MG1655) and the clinically isolated E. coli strains (EAEC, EIEC, EPEC, ETEC, A) by 12 antibiotic medicine (Fig S1A, S1B). Results are representative of at least 3 experiments in duplicates.

Article Snippet: Mueller-Hinton Broth was purchased from OXOID(Hampshire,U.K;Chemicals from Sigma (USA), EZMTT purchased from JNF Bioscience (China; USA), CCK-8 or WST-8 from Beyotime Biotechnology (China), Enterococcus casseliflavus Collins et al. ATCC700327, Escherichia coli (Migula) Castellani and Chalmers ATCC 25922,Pseudomonas aeruginosa (Schroeter) Migula ATCC 27853,Staphylococcus aureus subsp. aureus Rosenbach ATCC 25923 were purchased from ATCC (USA).

Techniques: Isolation

Journal: Journal of applied microbiology

Article Title: Bacterial inactivation by high-voltage atmospheric cold plasma: influence of process parameters and effects on cell leakage and DNA.

doi: 10.1111/jam.12426

Figure Lengend Snippet: Figure 4 Agarose gel electrophoresis showing genomic DNA and PCR-amplified products of untreated and ACP-treated samples. Voltage: 70 kVRMS; treatment time: 0–30 s; post-treatment storage time: 24 h; gas mix: air. Genomic DNA damage of (a) Escherichia coli ATCC 25922; (b) E. coli NCTC 12900; (c) Listeria monocytogenes NCTC 11994. 16S rRNA PCR results of (d) E. coli ATCC 25922; (e) E. coli NCTC 12900; (f) L. monocytogenes NCTC 11994. Lane 1: Nonplasma treatment control; 2: 5 s directly treated samples; 3: 5 s indirectly treated samples; 4: 30 s directly treated samples; 5: 30 s indirectly treated samples.

Article Snippet: E. coli ATCC 25922 populations were reduced below detection level after 30 s of indirect ACP treatment; however, direct ACP-treated population reduced by 3 4 log cycles.

Techniques: Agarose Gel Electrophoresis, Control